Figure 3.

Histological analysis of early seed development under control and severe and moderate heat stress conditions. Seeds were harvested at 24, 48, 72, and 96 HAF for control and severe stress samples. For moderate stress, seeds were collected at 48- and 72-HAF time points. A to D, Seeds from control condition plants (28°C). Endosperm (ed) cellularization was initiated by 72 HAF and completed by 96 HAF. E to G, Sections were obtained from plants exposed to a severe heat stress of 42°C initiated at 24 HAF for 2 d. Cellularization was delayed under severe heat stress, and the central vacuole (cv) was visible at 96 HAF. H and I, Under moderate heat stress (34°C), seeds exhibited early cellularization, and the endosperm was cellularized by 72 HAF. rms, Radical microtubule system. Bars = 200 µm.