Figure 3.

Expression of defense genes in atlyk3 mutants inoculated with B. cinerea. Wild-type (WT; empty bars), atlyk3-1 (light-gray bars), and atlyk3-3 (dark-gray bars) rosette leaves were inoculated with B. cinerea, and total RNA was extracted at the indicated times. Expression of PAD3 (A), PDF1.2 (B), and PR1 (C) was analyzed by qPCR using UBQ5 as reference. Bars indicate average expression ± sd of three independent biological replicates. Asterisks indicate significant differences between the wild type and mutants, according to Student’s t test (*P < 0.05, **P < 0.02, ***P < 0.01).