Figure 7.

Responses to OGs in atlyk3 mutants. A and B, Rosette leaves of adult wild-type (WT), atlyk3-1, and atlyk3-3 plants were infiltrated with water or 100 μg mL^–1 OG. Leaves were harvested after 24 h, and callose deposits were stained with aniline blue. A, Representative pictures of treated leaves obtained with a fluorescence microscope. B, Quantification of callose accumulation. Bars represent average fluorescence ± sd of at least six different samples. Asterisks indicate significant differences between water- and OG-treated samples, according to Student’s t test (*P < 0.05, ***P < 0.01). #, Significant difference between wild-type and mutant leaves treated with OGs, according to Student’s t test (P < 0.05). This experiment was repeated twice with similar results. C, Expression of PAD3 was analyzed by qPCR in wild-type, atlyk3-1, and atlyk3-3 liquid-grown seedlings treated for 3 h with water or 100 μg mL^–1 OGs. The UBQ5 gene was used as reference. Bars indicate average expression ± sd of three independent biological replicates. Asterisks indicate significant differences between water- and OG-treated samples, according to Student’s t test (*P < 0.05, ***P < 0.01). D, Four-week-old wild-type and atlyk3-1 plants were sprayed with water or 200 μg mL^–1 OGs, and after 24 h, they were inoculated with B. cinerea. Lesion size was measured at 48 h post infection. Bars indicate average lesion area ± se of at least 12 lesions. Different letters indicate statistically significant differences, according to one-way ANOVA followed by Tukey’s honestly significant difference test (P ≤ 0.01). This experiment was repeated three times with similar results.