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. 2014 Mar 24;165(1):290–308. doi: 10.1104/pp.113.233742

Figure 1.

Figure 1.

Synthesis and accumulation of GlcNAc monomers and oligomers in NodC OE lines. A, NodC catalyzes the transfer of GlcNAc from UDP-GlcNAc to the growing GlcNAc oligomer. B, Retention times of the GlcNAc monomer as well as the different GlcNAc oligomers (DP = 2–6) as determined by authentic reference compounds. During derivatization, each compound is labeled with two 1-phenyl-3-methyl-5-pyrazolone molecules, causing a shift of 331 in positive mode compared with its nominal mass. C, MS spectrum of (GlcNAc)6 as a representative example. The degradation products corresponding to (GlcNAc)n compounds with different DP (n = 1–5) are indicated. D, Quantification of (GlcNAc)n compounds (n = 1–5) by ultra-performance liquid chromatography-MS in NodC OE lines (pTGK42-10 and pTGK42-28) and the wild type (WT). Values are means ± sd.