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. 2014 Mar 24;165(1):290–308. doi: 10.1104/pp.113.233742

Figure 7.

Figure 7.

Microscopic analyses of transverse stem sections of the NodC OE line pTGK42-28 (right) compared with the wild type (WT; left). A, Scanning electron microscopy images of a 250-µm vibroslice stem section revealing syncytium-like structures in the pith region of NodC OE lines. Bars = 200 µm. B, Detail of A. Bars = 200 µm. C, Toluidine blue-stained semithin stem sections illustrating that ruptured cell walls are at the base of the observed modifications in the pith region. Arrowheads point to modified pith cells. Bars = 100 µm. D, Transmission electron microscopy cross sections confirming the presence of ruptured cell wall in pith cells of NodC OE lines. Bars = 10 µm. Similar defects were observed in pTGK42-10 lines.