Figure 1.

Identification of salt-tolerant COS-transformed Arabidopsis calli. A, Screening of COS-transformed cell culture on MS medium containing 175 mm NaCl and 5 µm estradiol. B and C, Growth of transformed line number 1 on high-salt medium without (B) and with estradiol (C). D and E, Wild-type Arabidopsis calli on high-salt medium in the absence (D) or presence (E) of estradiol. F and G, pER8GW-HSFA4A-transformed Arabidopsis colonies growing on high-salt medium containing 5 µm estradiol. Transformation frequency was approximately 5% in this experiment. H, Transformed cells are unable to grow on high-salt medium in the absence of estradiol. Bar = 5 mm. [See online article for color version of this figure.]