HSFA4A overexpression reduces oxidative damage during salt stress. A, H2O2 accumulation in Col-0 wild-type, HSFA4ox1, and HSFA4ox2 plants treated with 150 mm NaCl for 6 and 24 h. B, Lipid peroxidation in salt-stressed plants calculated by malondialdehyde accumulation. One hundred percent corresponds to lipid damage in wild-type nonstressed plants. C, APX activities in salt-treated plants. In vitro-grown plantlets were used for the experiments at age of 14 d. Error bars indicate ses, and different letters show significant differences at P < 0.05 (Duncan’s test). Five micromolar estradiol was included in all treatments. FW, Fresh weight.