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. 2014 Mar 27;165(1):319–334. doi: 10.1104/pp.114.237891

Figure 5.

Figure 5.

HSFA4A regulates the expression of a set of stress-related genes. A, Transcript analysis of eight HSFA4A target genes in HSFA4ox2 and Col-0 wild-type plants using qRT-PCR: HSP17.6A (AT5G12030), CTP1 (AT5G52760), ZAT6 (AT5G04340), ZAT12 (AT5G59820), WRKY30 (AT5G24110), CRK13 (AT4G23210), ATL31 (AT5G27420), and IPMI1 (AT3G58990). Plants were treated with 1 mm H2O2 or heat stress (37°C) for 6 h. All plants were treated with 5 μm estradiol. B and C, HSFA4A-regulated genes can be induced by salt. B, Expression of selected HSFA4A-regulated genes in HSFA4ox2 and Col-0 plants in control conditions (Ctr) or treated with 100 mm NaCl for 6 h (all plants were treated with 5 μm estradiol). C, Expression of the selected genes in hsfa4a mutant and wild-type plants (ecotype Wassilewskija [Ws]) in control conditions (Ctr) or treated with 100 mm NaCl for 6 h. Plants were 2 weeks old when subjected to treatments; whole seedlings where used. Transcript abundance was calculated with the 2−ΔΔCt method according to Fohgrub and Kempken (2012).