Figure 2.

OsAHP-RNAi seedlings show reduced sensitivity to exogenous cytokinin (CK). A and B, The inhibitory effects of exogenous trans-zeatin on lateral root generation. Roots of 10-d-old seedlings were grown on 1/2 MS medium with or without the indicated concentrations of zeatin (A); the lateral root number produced on the seminal root (1.5 cm in length) in each seedling was counted (B). Error bars represent sd (n = 12). Bars = 5 mm. C and D, The delayed effects of exogenous BA on dark-induced leaf senescence. Leaves were treated with or without BA in the dark for 45 h (C) and then analyzed for chlorophyll retention (D). Two parallel samples for each treatment were measured, and three repeats were performed. The data are given as means ± sd. FW, Fresh weight. E, Expression of type A RR genes in response to BA treatment. Twelve-day-old seedlings were used for qRT-PCR assays; the mRNA level from wild-type (WT) plants without BA treatment was set at 1. Os18S was used as an internal control. The data are given as means ± sd of three biological replications. Asterisks indicate that the value is significantly different from the value for the wild type as determined by Student’s t test (***P < 0.001).