Figure 3.

METE is expressed in the presence of vitamin B₁₂ in C. subellipsoidea, which lacks METH. A, Comparison of the METE amino acid sequences of C. subellipsoidea (C.s), C. merolae (C.m), and P. tricornutum (P.t) with the C. reinhardtii (C.r) METE antibody-binding site (Schneider et al., 2008). Shared residues are shaded gray. B, METE abundance in C. subellipsoidea in the presence (10 µg L⁻¹) and absence of vitamin B₁₂ using western blotting with the C. reinhardtii METE antibody (Schneider et al., 2008). C, Quantification of METE levels in C. subellipsoidea in the presence (10 µg L⁻¹) and absence of B₁₂. Values are means and se (n = 3).