Table I. Normalization of mutation rates by cell number and ploidy per nucleus.
Different Arabidopsis hybrids and the Col × Col control were analyzed for their adaxial epidermal cell count and their ploidy per leaf cell nucleus to obtain the correction factor. The coefficient of interquartile range (CIQR) was calculated as a nonparametric measurement of variance in the style of the coefficient of variance. The relative number of cells and the relative ploidy are the normalization values for the hybrid, normalized to the Col × Col control. The correction factor was calculated by multiplying the two normalization values. This correction factor was used to correct the number of GUS spots before analysis. n, Number of plants analyzed; Median, median of measurements; CIQR, interquartile range/median.
| Cross | Epidermal Cells |
Ploidy Per Nucleus |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| n | Median | CIQR | Relative No. of Cells | n | Median | CIQR | Relative Ploidy | Correction Factor | |
| Col × Col | 14 | 36,616 | 0.465 | 1.000 | 4 | 4.12 | 0.064 | 1.000 | 1.000 |
| Col × C24 | 14 | 41,047 | 0.234 | 1.121 | 13 | 4.03 | 0.081 | 0.977 | 1.096 |
| C24 × Col | 14 | 45,671 | 0.234 | 1.247 | 14 | 4.15 | 0.273 | 1.007 | 1.256 |
| Col × Cvi | 12 | 38,081 | 0.063 | 1.040 | 20 | 3.94 | 0.104 | 0.957 | 0.996 |
| Cvi × Col | 27 | 63,175 | 0.317 | 1.725 | 21 | 4.32 | 0.028 | 1.048 | 1.808 |
| Col × Ws | 10 | 45,933 | 0.219 | 1.254 | 10 | 3.79 | 0.036 | 0.919 | 1.153 |
| Ws × Col | 11 | 55,158 | 0.527 | 1.506 | 10 | 3.31 | 0.047 | 0.804 | 1.211 |
| Col × Ler | 9 | 59,745 | 0.198 | 1.632 | 5 | 4.02 | 0.074 | 0.976 | 1.593 |
| Ler × Col | 9 | 69,330 | 0.346 | 1.893 | 12 | 4.17 | 0.048 | 1.013 | 1.919 |