Oxygen evolution rates of Synechocystis sp. strain PCC 6803 cultures in increasing intensities of 625-nm light, recorded with MIMS. 18O2 was added at the start of the experiment to monitor oxygen consumption in the light. Depicted are net photosynthesis (which is the sum of the measured oxygen production and the consumption [diamonds]) and respiration (squares). A, BG-11 with 0.5 mm NaHCO3. B, BG-11 with 50 mm NaHCO3. Orange symbols, 625-nm light only; red symbols, 625-nm light + 25 µmol photons m−2 s−1 730-nm light. Data shown are averages of biological duplicates with sd. [See online article for color version of this figure.]