Figure 1.

Extraction of protein and RNA from M and BS cells of S. viridis. A, Representative image of a leaf prior to rolling, with alternating bands of M (arrowhead) and BS cells. B, After leaf rolling, the chloroplasts within the BS cells (circle) are visible. C, Representative image of BS preparation after blending. D, Immunoblotting demonstrates that CA, PEPC, and NADP-MDH proteins were abundant in M samples but not detectable in BS strands. In contrast, Rubisco LSU and NADP-ME were preferentially localized in BS strands. The molecular mass of each protein is annotated to the left of each blot. E, Quantitative PCR for CA, PEPC, NADP-MDH, RbcS, and NADP-ME indicated preferential transcript accumulation in the same cell type as each protein. Bars = 200 µm (A and B) and 4 µm (C).