Table II. Abundance of transcripts encoding enzymes required for the C4 pathway in M or BS cells of S. viridis.
Each gene recruited into the C4 pathway in S. viridis is highly expressed and preferentially generates transcripts in either M or BS cells. Proteins are listed in groups according to whether they function in the core C4 pathway, metabolite transport, the Calvin-Benson cycle, or photorespiration. Library-normalized read counts, fold enrichment in each cell type, and Benjamini-Hochberg procedure adjusted P values are shown.
| Gene | Identifier | Role | Cell Type | M | BS | Fold Change, BS/M | Log2 Fold Change, BS/M | Adjusted P |
|---|---|---|---|---|---|---|---|---|
| CA | Si003882m.g | Core pathway | M | 453,421.09 | 12,315.93 | 0.03 | −5.20 | 5.23E-093 |
| PEPC | Si005789m.g | Core pathway | M | 1,413,131.82 | 47,109.98 | 0.03 | −4.91 | 7.31E-060 |
| NADP-MDH | Si013632m.g | Core pathway | M | 108,641.70 | 4,588.13 | 0.04 | −4.57 | 5.21E-076 |
| PPDK | Si021174m.g | Core pathway | M | 1,169,325.81 | 57,155.39 | 0.05 | −4.35 | 5.61E-071 |
| AK | Si014186m.g | Core pathway | M | 99,257.32 | 12,079.26 | 0.12 | −3.04 | 1.91E-039 |
| ASP-AT | Si017156m.g | Core pathway | M | 74,263.60 | 10,050.17 | 0.14 | −2.89 | 4.34E-036 |
| PPDK-RP | Si032116m.g | Core pathway | M | 17,359.84 | 7,810.30 | 0.45 | −1.15 | 3.20E-007 |
| NADP-ME | Si000645m.g | Core pathway | BS | 4,155.43 | 613,075.40 | 147.54 | 7.20 | 7.82E-084 |
| RBCS | Si023465m.g | Core pathway | BS | 2,717.93 | 290,283.67 | 106.80 | 6.74 | 6.79E-103 |
| PCK | Si034404m.g | Core pathway | BS | 240.11 | 3,170.30 | 13.20 | 3.72 | 3.18E-051 |
| RBCACT | Si026402m.g | Core pathway | BS | 6,245.84 | 77,396.56 | 12.39 | 3.63 | 3.21E-021 |
| OMT1 | Si024403m.g | Transport | M | 32,208.32 | 1,289.06 | 0.04 | −4.64 | 2.17E-077 |
| MEP3b | Si000451m.g | Transport | M | 63,801.82 | 10,077.02 | 0.16 | −2.66 | 2.68E-029 |
| PPT | Si013874m.g | Transport | M | 64,440.52 | 17,850.85 | 0.28 | −1.85 | 3.81E-016 |
| DIT1 | Si029415m.g | Transport | M | 3,703.36 | 2,349.68 | 0.63 | −0.66 | 9.64E-003 |
| DCT2 | Si035016m.g | Transport | BS | 650.50 | 137,421.82 | 211.25 | 7.72 | 3.68E-157 |
| MEP3a | Si024315m.g | Transport | BS | 675.13 | 11,279.22 | 16.71 | 4.06 | 1.93E-011 |
| TPI | Si030636m.g | Calvin cycle | M | 52,985.29 | 3,153.93 | 0.06 | −4.07 | 1.39E-063 |
| GAPDH | Si035707m.g | Calvin cycle | M | 230,052.78 | 124,502.17 | 0.54 | −0.89 | 1.41E-004 |
| PRK | Si017390m.g | Calvin cycle | BS | 1,348.39 | 210,577.18 | 156.17 | 7.29 | 2.86E-065 |
| FBA | Si010312m.g | Calvin cycle | BS | 4,611.67 | 616,786.63 | 133.74 | 7.06 | 6.1E-088 |
| SBP | Si001775m.g | Calvin cycle | BS | 1,174.47 | 134,922.00 | 114.88 | 6.84 | 1.20E-059 |
| RPI | Si030780m.g | Calvin cycle | BS | 436.32 | 24,891.21 | 57.05 | 5.83 | 2.01E-088 |
| RPE | Si036994m.g | Calvin cycle | BS | 1,528.85 | 70,559.89 | 46.15 | 5.53 | 5.86E-035 |
| FBP | Si035941m.g | Calvin cycle | BS | 3,018.51 | 56,039.35 | 18.57 | 4.21 | 2.46E-032 |
| TKL | Si005927m.g | Calvin cycle | BS | 24,364.05 | 131,028.52 | 5.38 | 2.43 | 8.46E-010 |
| PGK | Si021917m.g | Calvin cycle | BS | 59,178.50 | 87,873.79 | 1.48 | 0.57 | 3.05E-002 |
| GDC | Si000068m.g | Photorespiration | BS | 388.71 | 74,572.76 | 191.85 | 7.58 | 1.48E-103 |
| GCH | Si011200m.g | Photorespiration | BS | 65.68 | 9,987.76 | 152.06 | 7.25 | 3.05E-136 |
| SHMT | Si035240m.g | Photorespiration | BS | 3,133.86 | 60,501.53 | 19.31 | 4.27 | 7.07E-067 |
| GOX | Si040072m.g | Photorespiration | BS | 10,487.89 | 23,729.01 | 2.26 | 1.18 | 4.71E-007 |