Table I. Proteins with potentially changed abundance in ethe1-1 mitochondria.

Mitochondrial proteins from wild-type and ethe1-1 cell culture were separated by IEF/SDS-PAGE (Supplemental Fig. S3). Gels from three biological replicates were analyzed by Delta2D (Decodon), and the proteins in spots with at least 1.5-fold changes in volume were identified by nano-liquid chromatography-tandem mass spectrometry. For additional information, see Supplemental Table S2.

Spot	Accession No.	Name	Ratioa	Spot	Accession No.	Name	Ratioa
1	AT3G55410	E1 2-oxoglutarate dehydrogenase	4.23	25	AT1G51390	NifU5	0.66
2	AT3G61440	β-Cyanoalanine synthase	3.98	26	AT5G07440	Glu dehydrogenase	0.63
3	AT2G05710	Aconitase	3.06	27	AT1G65290	Acyl carrier protein	0.63
4	AT3G10920	Superoxide dismutase	2.92	28	AT1G06530	Tropomyosin-related protein	0.63
5	AT3G55410	E1 2-oxoglutarate dehydrogenase	2.90	28	AT4G26780	Chaperone	0.63
6	AT2G29530	Tim10 (for 10-kD subunit of the preprotein translocase of the inner mitochondrial membrane)	2.54	29	AT2G30970	Asp aminotransferase	0.62
7	AT2G45060	Hypothetical protein	2.41	30	AT3G20970	NifU4	0.61
7	AT1G53240	Malate dehydrogenase	2.41	31	AT2G28000	Chaperonin 60α	0.61
7	AT2G30920	Hexaprenyl methyltransferase	2.41	31	AT3G02650	TPR-like protein	0.61
7	AT3G18580	Single-stranded DNA-binding protein	2.41	32	AT4G35090	Catalase	0.61
7	AT5G13450	ATP synthase subunit O	2.41	33	AT5G62690	Tubulin	0.60
7	AT4G26910	E2 2-oxoglutarate dehydrogenase	2.41	34	AT5G66760	Succinate dehydrogenase	0.60
7	AT5G23140	Caseinolytic protease	2.41	35	AT2G07698	ATP synthase subunit α	0.58
8	AT5G18170	Glu dehydrogenase	2.41	35	AT1G48030	E3 dihydrolipoyl dehydrogenase	0.58
9	AT5G26780	Ser hydroxymethyltransferase	2.38	36	AT2G43400	ETFQO	0.58
9	AT5G62530	δ-Pyrroline-5-carboxylate dehydrogenase	2.38	37	AT3G58610	Ketol-acid reductoisomerase	0.55
10	AT2G42210	Tim17 domain-containing protein	2.36	38	AT2G33210	Chaperonin CPN60	0.53
11	AT4G34030	Methylcrotonyl-CoA carboxylase	2.17	38	AT5G04740	ACT domain-containing protein	0.53
12	AT3G06850	E2 branched-chain α-keto acid dehydrogenase	2.04	39	AT2G43090	3-Isopropylmalate dehydratase	0.50
13	AT3G13930	E2 pyruvate dehydrogenase	2.01	40	AT3G23990	Chaperonin CPN60	0.49
14	AT5G65750	E1 2-oxoglutarate dehydrogenase	1.96	41	AT3G43810	Calmodulin	0.37
14	AT2G22780	Malate dehydrogenase	1.96	42	AT1G22300	General regulatory factor10 (GRF10; 14-3-3-like)	0.37
15	AT3G02780	Isopentenyl-diphosphate δ-isomerase	1.89	42	AT5G38480	GRF3 (14-3-3-like)	0.37
15	AT2G21870	ATP synthase subunit	1.89	42	AT4G22240	Lipid-associated protein	0.37
16	AT4G05400	Copper ion-binding protein	1.84	43	AT1G54580	Acyl carrier protein	0.29
17	AT4G34700	Complex I β-subcomplex 9	1.83	44	AT4G24280	Heat shock 70-kD protein (HSP70-1)	0.28
18	AT5G08530	Complex I flavoprotein1	1.73	45	AT1G66410	Calmodulin	0.24
18	AT2G07698	ATP synthase subunit α	1.73
19	AT3G13110	Ser acetyltransferase	1.66
19	AT3G26760	Rossmann fold-containing protein	1.66
19	AT5G20080	NADH-cytochrome b5 reductase	1.66
19	AT5G63400	Adenylate kinase	1.66
20	AT5G66510	γ-Carbonic anhydrase	1.64
20	AT5G43430	Electron transfer flavoprotein	1.64
20	AT5G15090	VDAC3 (for Mitochondrial outer membrane protein porin3)	1.64
21	AT2G05710	Aconitase	1.62
21	AT5G08670	ATP synthase subunit β	1.62
21	AT3G17240	E3 dihydrolipoyl dehydrogenase	1.62
21	AT1G63940	Monodehydroascorbate reductase	1.62
22	AT3G18580	Single-stranded DNA-binding protein	1.62
22	AT2G31140	Peptidase	1.62
22	AT4G15640	Unknown protein	1.62
22	AT4G15940	Fumarylacetoacetate hydrolase	1.62
22	AT5G13450	ATP synthase subunit O	1.62
23	AT5G65720	Cys desulfurase (NifS)	1.60
24	AT5G26780	Ser hydroxymethyltransferase	1.53

^aRatio of mean spot volume on ethe1-1 gels to mean spot volume on wild-type gels.