Extended Data Figure 5. Coordinated function of Ascl2 and Batf/IRF4 in regulating Tfh-related genes.
(ChIP-Seq data of Maf, Batf, and IRF4 were derived from GSE40918 25)
a. Venn diagram of ChIP-Seq peaks from Ascl2 and Maf.
b. Distribution of ChIP-Seq peaks by Ascl2, Batf, and IRF4 on gene loci including Bcl6, Prmd1, Ascl2 and Maf.
c. Venn diagram of ChIP-Seq peaks from Ascl2, Batf and IRF4.
d. Distribution of ChIP-Seq peaks by Ascl2, Batf, and IRF4 on gene loci including Cxcr5, Cxcr4, Ccr7, Selplg1, Il2, Il2ra, and Il2rb. Blue frame represents the co-localization of peaks.
e. Distribution of ChIP-Seq peaks by Ascl2, Batf, and IRF4 on Th1-related Tbx21 and Ifng gene loci.
f. Distribution of ChIP-Seq peaks by Ascl2, Batf, and IRF4 on Th2-related Gata3, Il4, Il13 and Il5 gene loci.
g. Distribution of ChIP-Seq peaks by Ascl2, Batf, and IRF4 on Th17-related Rorc, Il17a, Il17f and Il21 gene loci.
ChIP-Seq assay of Ascl2 were performed on Ascl2-overexpressed T cell cultured under Th0 condition. ChIP-Seq assay of Maf, Batf and IRF4 were performed on Th0 cells by Dan R. Littman’s group, derived from GSE40918 25.