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. Author manuscript; available in PMC: 2015 May 1.
Published in final edited form as: Inflamm Bowel Dis. 2014 May;20(5):835–846. doi: 10.1097/MIB.0000000000000033

Fig. 7. CHI3L1 synergistically enhances the IL-6-mediated STAT3-phosphorylation in CHI3L1 KO mice-derived colonic crypt cells.

Fig. 7

(A) Colonic crypt cells were freshly isolated from 8–10 weeks old CHI3L1 KO mice and stimulated with recombinant mouse IL-6 (100 ng/ml), recombinant mouse IL-22 (10 ng/ml) and/or purified human CH3L1 (80 ng/ml) for 15 minutes at 37°C with 5% CO2. After stimulations, the samples were lysed and 30 µg/lane of protein lysate was subjected to immunoblot with anti-phospho (P) or anti-total (T) -STAT3 antibodies. (B) Densitometry analysis of phospho-STAT3 signals (left) and phospho-STAT3 signals normalized to total STAT3 signals (right) is shown. *P<.05, **P<.01, and NS, no significant difference (versus control or between the indicated groups).