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. Author manuscript; available in PMC: 2014 May 7.
Published in final edited form as: J Pathol. 2011 Dec 9;226(4):633–644. doi: 10.1002/path.3016

Figure 5.

Figure 5

TGFβ1 receptor inhibitor SB431542 blocks induction of α-SMA by type I collagen. MAECM grown on type I collagen exhibit irregular and disorganized morphology (A) and a subset of GFP-labeled cells express α-SMA (B; magenta). MAECM grown on type I collagen in the presence of SB431542 (10 μM) displayed cobblestone-like morphology and GFP-labeled cells did not express α-SMA (D), indicating prevention of collagen-induced EMT. GFP-labeled cells grown on laminin-5 with or without SB431542 retain a cobblestone-like morphology (E, G) and showed no immunoreactivity for α-SMA (F, H). Nuclei were counterstained with DAPI (blue). Original magnification x20. I: MAECM were trypsinized off type I collagen- and laminin-5-coated polycarbonate filters and GFP-positive cells were purified by FACS. Lysates of GFP-positive cells were subjected to SDS-PAGE and immunoblotted with mouse monoclonal anti-α-SMA antibody. Lamin A/C was used as a loading control. Data shown are representative of 3 separate experiments.