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. Author manuscript; available in PMC: 2014 May 7.
Published in final edited form as: J Pathol. 2011 Dec 9;226(4):633–644. doi: 10.1002/path.3016

Figure 9.

Figure 9

. Pan-specific TGFβ neutralizing antibody blocked induction of α-SMA by exogenous TGFβ1. Wild-type MAECM grown on laminin-5 in the presence of exogenous TGFβ1 (2.5 ng/mL) expressed α-SMA (C-D; red) after 8 days in culture, whereas in the absence of TGFβ1 (A-B), in the presence of TGFβ1 and the TGFβ neutralizing antibody (E-F), or with TGFβ neutralizing antibody alone (G-H), MAECM have minimal or no α-SMA reactivity. Original magnification x20. Panels B, D, F and H show magnified views of the rectangles shown in panels A, C, E and G, respectively, after cropping and re-sizing using Adobe Photoshop. I: Wild-type MAECM grown on laminin-5 develop RT > 2 kΩ·cm2 on day 4. Representative experiment demonstrates that exogenous TGFβ1 reduced RT to near 0 kΩ·cm2 on day 8; the TGFβ neutralizing antibody blocked the TGFβ1-induced reduction in RT.