Primer set | Sequence (5'→3') | Position in human genome (NCBI sequence accession no.) | Annealing temp (°C) |
---|---|---|---|
B48-F | CTCCACCCCCAAGGAAAAAG | 2368081-2368062 (NT_011255.14) | 60 |
B48-R | GGCAGGGTCCCATGCA | 2368005-2368020 (NT_011255.14) | |
B23-F | GTCCACCTCACTAATGCAGACAAT | 33836142-33836165 (NT_ 034772.5) | 60 |
B23-R | AGAGAAGCAGGAAGGGCTTAGAGA | 33836213-33836193 (NT_ 034772.5) | |
B17-F | AGCCAAGGTGCCTCTTTCAG | 33833183-33833202 (NT_ 034772.5) | 60 |
B17-R | GTCCGGGAGGAGCAGACA | 33833240-33833223 (NT_ 034772.5) | |
B13-F | CCCCAGGGAGTAGGTTGTGA | 2363991-2363972 (NT_011255.14) | 60 |
B13-R | TGTTATTTGAGAAAAGCCCAAAGAC | 2363892-2363916 (NT_011255.14) | |
Myc2-F | CTTATGCCTCTATCATTCTTCCT | 1776-1795 (X00364) | 56 |
Myc2-R | TAAATCATCGCAGGCGGAAC | 1981-2000 (X00364) | |
MCM4-F | AAACCAGAAGTAGGCCTCGCTCGG | 12946-12969 (U63630) | 58 |
MCM4-R | GTCTGACCTGCGGAGGTAGTTTGG | 13460-13482 (U63630) |