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. 2014 Mar 12;5(5):1363–1381. doi: 10.18632/oncotarget.1734

Figure 5. HIF-1α expression are involved in IMQ-induced apoptosis in tumor cells.

Figure 5

(A and B) The knock-down of HIF-1α sensitized tumor cells to IMQ-induced apoptosis. (A) BCC and SCC12 cells were transfected with a HIF-1α specific siRNA or a non-specific siRNA for 24 hours and then treated with 50 μg/ml IMQ for 24 or 48 hours, respectively. The cells were then analyzed with a DNA content assay based on PI staining and flow cytometry. (B) The siRNA transfected BCC cells incubated with 50 μg/ml IMQ for 12 hours were analyzed by immunoblotting with cleaved-PARP, cleaved-caspase 9, cleaved-caspase 3, Mcl-1 and β-actin antibodies. The data are expressed as the mean ± S.E.M. of at least three independent experiments. * p < 0.05; ** p < 0.01.