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. 2014 Jan 12;5(5):1253–1264. doi: 10.18632/oncotarget.1690

Figure 1. ADAMTS-12 and fibulin-2 are two interacting proteins.

Figure 1

(A) Schematic illustration of the interacting regions between ADAMTS-12 and fibulin-2 determined by yeast two-hybrid screen. TSP-1, thrombospondin-1-like domain; Cys-rich domain, cysteine-rich domain; PLAC, protease-lacunin; RGD, Arginine-Glycine-Aspartic Acid; EGF, ephitelial growth factor; CB-EGF, Calcium-binding EGF; BD, binding domain; AD, activating domain. (B) Western blot analysis of immunoprecipitated samples. Top, 293-EBNA control cells extracts (EBNAc) or 293-EBNA cell extracts producing ADAMTS-12-FLAG (EBNA-ADAMTS-12) were incubated with the indicated amounts of fibulin-2, immunoprecipitated with using anti-FLAG M2 affinity gel and further detection was performed with an anti-fibulin-2 antibody. Bottom, cell extracts were immunoprecipitated with an anti-fibulin-2 antibody and detection was performed with an anti-ADAMTS-12 antibody. F indicates that 1 μg of fibulin-2 was loaded in these lanes. Molecular weight marker is indicated on the right.