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. 2014 Jan 12;5(5):1253–1264. doi: 10.18632/oncotarget.1690

Figure 4. Simultaneous production of fibulin-2 and ADAMTS-12 reduces self-renewal of mammosphere-forming units in MCF-7 cells.

Figure 4

(A) Representative images of mammospheres derived from MCF-7-(fb) (fibulin-2), MCF-7-(ts) (ADAMTS-12), and MCF-7-(fb/ts) (ADAMTS-12/fibulin-2). Control, MCF-7 cells transfected with an empty vector. Sizes of some mammospheres are indicated. (B) Mammospheres were dissociated and passaged at a density of 20 cell/well in 96-well plates, and MFUs were counted and calculated as a percentage of mammospheres formed from the number of cell seeded.