Figure 3. The transformed cells induced by As³⁺ express genes for the stemness of CSCs.

(A) Gene expression profiles were compared between parental (BEAS-2B) and the transformed (Transf) cells using Affymetric Human Gene 1.0 ST Arrays. (B) Ingenuity Pathway assay for the differentially expressed genes in the transformed cells compared to the parental cells based on BioRank Scores. (C) Verification of the differentially expressed genes by real-time PCR. (D) Western blotting to determine the expression levels of the transcription factors and proteins important for the self-renewal and pluripotency of the CSCs between parental (BEAS-2B) and the transformed (Transf) cells.