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. 2014 Mar 16;5(5):1212–1225. doi: 10.18632/oncotarget.1831

Figure 2. Expression of mutant p53 regulate CIC levels.

Figure 2

A. Lane 1, H1299 transfected with control vector; lane 2: transfection with the wt-p53 expressing vector; lane 3: transfection with the p53R175H vector. Cell lysates derived from these transfection experiments were subjected to immuno-blot with the anti-p53 (upper panel); with the anti-CIC (second panel), or with anti-actin antibodies (lower panel). B. CIC levels in H1299 cells or in H1299 cells stably expressing the p53 mutants indicated at the top of the panel. C. CIC expression levels in isogenic cell lines derived from murine mammary tumor cancers. Three cell lines were used, p53-/- (lane 1), p53 wild-type (lane 2) or cells harboring a mutation at position 245 that replaces a glycine with an alanine (p53A242G; lane 3). D-E. MDA-231 (D) or MDA-468 cells (E), harboring a tetracycline-inducible vector (tet-off) expressing the p53 shRNA were grown in the presence of doxycycline (p53shRNA off), or in its absence (p53shRNA on), cell extracts were prepared and analyzed as described before.