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Invasive necrosis quantification. Viable tumor is selected via histological pattern recognition software (Genie; Aperio) in green. Other tissues are classified in red and necrotic tissues are classified as blue. In the top panel A) and B) and ER + sample is presented in comparison to C) and D) in which an ER- sample is shown. Area of necrosis as a percentage of total tumor area (viable tumor and necrotic region) data for each sample is presented. Scale bar = 5 mm.
