Table 5. PCR results for detection of BLV in breast tissue samples from 6 women*.
| Sample code | Sample pathology | Patient age, y | BLV genome regions |
||||||
|---|---|---|---|---|---|---|---|---|---|
| L-PCR |
IS-PCR |
||||||||
| LTR | gag | pol | env | tax | tax | ||||
| 143 | Malignant | 63 | – | – | – | – | – | – | |
| 0253 | Malignant | 47 | + | – | – | – | + | + | |
| 010 | Malignant | 48 | + | – | – | – | + | + | |
| 236 | Nonmalignant | 54 | + | + | – | + | + | + | |
| 23803 | Nonmalignant | 50 | + | + | – | + | + | + | |
| 20874 | Nonmalignant | 53 | + | + | + | + | + | + | |
*BLV, bovine leukemia virus; L-PCR, nested liquid-phase PCR; IS-PCR, nested in situ PCR; LTR, long terminal repeat (promoter region); gag, group-specific antigen (capsid region); pol, polymerase (reverse transcription); env, envelope; tax, transactivating region of the X gene.