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. 2014 Apr 7;13(5):2370–2380. doi: 10.1021/pr4011684

Figure 3.

Figure 3

. Differential expression of the mexEF–oprN operon explains differential chloramphanicol resistance in P. aeruginosa PAO1 and PA14 strains. (A) Protein expression levels of MexEF–OprN in PAO1 and PA14. Two biological replicates are plotted for each strain, as shown by the same color bars. In PA14, we could not detect MexF or OprN, and the protein abundance score for MexE was low (∼0.2). (B) mRNA levels of mexEF–oprN in PAO1 and PA14. Two biological replicates are plotted for each strain, as shown by the same color bars. (C) Chloramphenicol disk diffusion assay. Exponentially growing PAO1 and PA14 were swabbed on an agar plate and exposed to increasing levels of chloramphenicol. Three biological replicates were performed, and a representative is shown.