Table 1. Fourteen Genes with Significantly Different mRNA and Protein Abundances between PAO1 and PA14a.
| PAO1 identifier | PAO1 protein abundanceb | PA14 protein abundanceb | PAO1 mRNA abundancec | PA14 mRNA abundancec | annotation |
|---|---|---|---|---|---|
| PA0997|pqsB | 0.0 | 38.9 | 36.0 | 1934.0 | homologous to beta-keto-acyl-acyl-carrier protein synthase |
| PA0998|pqsC | 0.0 | 36.6 | 27.5 | 1003.0 | homologous to beta-keto-acyl-acyl-carrier protein synthase |
| PA2235|pslE | 4.2 | 0.0 | 844.5 | 32.0 | hypothetical protein |
| PA2493|mexE | 43.3 | 0.3 | 5211.0 | 307.0 | resistance-nodulation-cell division (RND) multidrug efflux membrane fusion protein MexE precursor |
| PA2494|mexF | 35.3 | 0.0 | 4251.5 | 246.0 | resistance-nodulation-cell division (RND) multidrug efflux transporter MexF |
| PA2495|oprN | 38.7 | 0.0 | 3620.0 | 186.0 | multidrug efflux outer membrane protein OprN precursor |
| PA2667 | 15.0 | 42.0 | 627.0 | 2043.0 | conserved hypothetical protein |
| PA2813 | 4.7 | 0.0 | 392.5 | 141.0 | probable glutathione S-transferase |
| PA4502 | 0.0 | 7.8 | 117.5 | 833.0 | probable binding protein component of ABC transporter |
| PA4771|lldD | 10.5 | 29.6 | 709.5 | 1772.5 | l-lactate dehydrogenase |
| PA4772 | 0.7 | 10.8 | 933.5 | 2008.0 | probable ferredoxin |
| PA4778 | 0.0 | 9.7 | 266.5 | 1392.5 | probable transcriptional regulator |
| PA5261|algR | 6.6 | 1.1 | 207.0 | 100.0 | alginate biosynthesis regulatory protein AlgR |
| PA5289 | 5.0 | 0.0 | 655.0 | 190.5 | hypothetical protein |
a
A comprehensive list of differentially expressed genes is available in Supporting Information Tables S1 and S2. A >2-fold-change and FDR<0.05 were applied to detect significant differences both in mRNA and protein.
b
The protein abundance value represents the average APEX score of two biological replicates.
c
The mRNA abundance value represents the average normalized microarray signals of two biological replicates.