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. Author manuscript; available in PMC: 2014 May 7.
Published in final edited form as: Nat Genet. 2012 Dec 9;45(1):83–87. doi: 10.1038/ng.2497

Figure 4. Fusion of LC3-positive puncta with lysosomes in Vici syndrome.

Figure 4

In control fibroblasts subjected to 6h bafilomycin treatment, lysosomal structures were detected by staining with monoclonal anti-LAMP1. Numerous LC3-positive autophagosomes are found engulfed by the LAMP1-positive vesicular structures (arrowheads). In contrast, Vici patients fibroblasts consistently show smaller LC3-positive puncta that only sporadically colocalise with LAMP1, with many isolated LC3-positive puncta (arrows). Note that LC3 signal in Vici cells occurs mostly at the rim of LAMP1-positive structures, not centrally. Scale bar: 5 μm