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. 2012 Jan 9;9(3):267–275. doi: 10.1038/cmi.2011.56

Figure 4.

Figure 4

Salmeterol decreases the mRNA and protein levels of pro-inflammatory cytokines in LPS-activated DCs. (a) Effect of salmeterol on mRNA levels of pro-inflammatory cytokines in LPS-activated DCs. Murine BMDCs (day 6) were treated as indicated, and then they were harvested and the total RNA was extracted. Real-time PCR was performed to examine relative levels of TNF-α, IL-1β and IL-6 mRNA expression. DCs (day 6) were treated with salmeterol (10 µM) and LPS (0.1 µg/ml) for 3 h and then analyzed using real-time PCR. *P<0.05; extra-LPS exposure group vs. salmeterol treatment group; data are representative of three independent experiments. (b) Effect of salmeterol on secretion of pro-inflammatory cytokines in LPS-activated DCs. Day 6 DCs were treated as indicated and were then analyzed for cytokine production by ELISA. The LPS concentration in the samples was 0.1 µg/ml. Samples were incubated with different concentrations of salmeterol for 9 h. *P<0.05; LPS (0.1 µg/ml) vs. LPS/salmeterol (10 µM). Data are representative of three independent experiments. BMDC, bone marrow-derived dendritic cell; DC, dendritic cell; LPS, lipopolysaccharide; TNF, tumor-necrosis factor.