Figure 5. CD45+ BMCs are recruited to the site of β cell injury upon VEGF-A induction and persist in islet remnants during β cell regeneration.

βVEGF-A mice were transplanted with GFP+ bone marrow, and 8 wks later VEGF-A was induced for 1 wk by Dox administration followed by 2 wks of Dox withdrawal; n=4–6 mice/time point. (A–C) Labeling for insulin (Ins, blue), CD31 (red) and GFP (green). (D–F) Labeling for insulin (Ins, blue), pan-hematopoietic marker CD45 (red) and GFP (green). Boxes in A–F denote enlargements in A′–F′. Scale bar in A is 50 μm and applies to A–F. Scale bar in A′ is 50 μm and applies to A′–F′.