Figure 2.

The effect of poly(I:C) on the synthesis of ICAM-1, VCAM-1 and M-CSF in human MCs. MCs were grown under standard culture conditions (basal) or incubated with poly(I:C) (10 μg/ml) for different intervals (3, 6, 12 or 24 h), and the synthesis of ICAM-1 (a, b), VCAM-1 (c, d) and M-CSF (e, f) was analyzed by real-time PCR (a, c, e) and FACS (b, d, f), as described in the section on ‘Materials and methods'. The results are given as the means±SEM of two experiments conducted in parallel for each condition. rRNA served as the reference gene. Comparable results were obtained in two independent series of experiments. ICAM-1, intercellular adhesion molecule 1; MC, mesangial cell; M-CSF, macrophage colony-stimulating factor; poly(I:C), polyriboinosinic:polyribocytidylic acid; real-time PCR, quantitative reverse transcriptase-polymerase chain reaction; VCAM-1, vascular cell adhesion molecule 1.