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. 2011 Mar 7;8(3):265–275. doi: 10.1038/cmi.2011.1

Figure 3.

Figure 3

Inhibition of LPS-induced TNF-α and IL-12 mRNA expression in THP-1 cells by MBL. THP-1 cells were stimulated with LPS (100 ng/ml) in the presence of the indicated concentrations of HSA, anti-MBL pAb and MBL, or MBL alone for 24 h. Samples were taken for RNA extraction from various groups, and the amplification and electrophoresis of TNF-α, IL-12p35 and IL-12p40 genes was carried out simultaneously. (a) Phosphorimage from an individual experiment representing three independent experiments. (b) Gray values of the DNA fragments. *P<0.05 as compared to the LPS-stimulated group. Similar results were observed in three independent experiments. β-actin was used as an internal control. HSA, human serum albumin; LPS, lipopolysaccharide; MBL, mannan-binding lectin; pAb, polyclonal antibody; TNF, tumor-necrosis factor.