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Effects of Cl− channel blockers on nitric oxide and prostanoids-induced relaxation in rat mesenteric arteries. Arteries were treated with 50 nmol/L apamin plus 50 nmol/L charybdotoxin and (A) 3 μmol/L indomethacin or (B) 100 μmol/L L-NAME for 30 min, followed by contraction by 1 μmol/L phenylephrine and then relaxation by acetylcholine (ACh). Experiments were performed without (control) or with 50 μmol/L NPPB or 100 μmol/L DIDS. Data were expressed as means±SEM (n=6).
