Figure 3.

Effects of caveolae-specific inhibitors on ox-LDL efflux by HUVECs. HUVECs were incubated with ox-LDL (40 μg/mL) for 24 h. After lipid loading, HUVECs were washed twice with PBS, and incubated for another 6, 12, and 24 h in an ox-LDL depleted medium with or without the caveolae inhibitors. After incubation, the cholesterol in the cytoplasm (A) and cholesterol in medium (B) were analyzed by HPLC. Similar results were obtained in six independent experiments. Data are mean±SD. ^bP<0.05 vs corresponding groups at 0 h.