Figure 4.

Effects of ox-LDL treatment and ox-LDL depletion on expression of caveolin-1 and LOX-1 in HUVECs. HUVECs in 75-cm² flasks were incubated with ox-LDL (40 μg/mL) for 0, 6, 12, and 24 h, followed by incubation with ox-LDL-depleted medium for 0, 6, 12, and 24 h. Total cell lysates were prepared at the various time points indicated. For each sample, 20 μg of lysate protein was used. Western immunoblotting assays using antibody against human β-actin and caveolin-1 and LOX-1 were conducted. Results were reproduced in three independent experiments. (A) HUVECs were incubated with ox-LDL for 0, 6, 12, and 24 h. (B) HUVECs were incubated with ox-LDL-depleted medium for 6, 12, and 24 after uptake of lipid by HUVEC.