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. 2010 Sep 13;31(10):1336–1342. doi: 10.1038/aps.2010.87

Figure 5.

Figure 5

Effect of silencing caveolin-1 on NF-κB translocation and LOX-1 expression in lipid-loaded HUVECs. HUVECs transfected with control siRNA or caveolin-1 siRNA were incubated with or without ox-LDL. Lysates were prepared after incubation for 24 h to determine the expression of NF-κB and LOX-1. Total cellular proteins from HUVECs transfected with control siRNA or siRNA for caveolin-1 were immunoblotted with the anti-caveolin-1, LOX-1, and β-actin antibodies, and nuclear proteins were immunoblotted with the anti-NF-κB. Similar results were obtained in six independent experiments. Data are mean±SD. bP<0.05 vs corresponding groups transfected with control siRNA and treated with ox-LDL. eP<0.05 vs corresponding groups transfected with control siRNA but not treated with ox-LDL.