Table 1. Effect of caveolae-specific inhibitors on DiI-ox-LDL permeability in human umbilical vein endothelial cells. Data represent means±SEM of four different experiments. bP<0.05 compared with controls.
Blank | Control | Carrangeen | Filipin | Nocodazole |
---|---|---|---|---|
0 | 63.4%±8.3% | 32.4%±7.7%b | 17.5%±7.4%b | 12.8%± 3.4%b |
Filter-grown HUVEC were cultured for 4 d to form a tight monolayer. HUVEC monolayer was exposed to DiI-ox-LDL for 24 h, or pretreated with caveolae inhibitors for 1 h followed by DiI-ox-LDL incubation. After incubation, samples were taken from the apical and basolateral compartment and measured in a fluorescence spectrophotometer. Values were expressed as % apical DiI-Ox-LDL per well that crossed the Transwell membrane.