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. 2010 Sep 13;31(10):1336–1342. doi: 10.1038/aps.2010.87

Table 1. Effect of caveolae-specific inhibitors on DiI-ox-LDL permeability in human umbilical vein endothelial cells. Data represent means±SEM of four different experiments. bP<0.05 compared with controls.

Blank Control Carrangeen Filipin Nocodazole
0 63.4%±8.3% 32.4%±7.7%b 17.5%±7.4%b 12.8%± 3.4%b

Filter-grown HUVEC were cultured for 4 d to form a tight monolayer. HUVEC monolayer was exposed to DiI-ox-LDL for 24 h, or pretreated with caveolae inhibitors for 1 h followed by DiI-ox-LDL incubation. After incubation, samples were taken from the apical and basolateral compartment and measured in a fluorescence spectrophotometer. Values were expressed as % apical DiI-Ox-LDL per well that crossed the Transwell membrane.