Figure 2.

Effect of matrine on cell cycle progress in cultured VSMCs. VSMCs were treated with various doses of matrine (0, 5, 10, 15, and 20 mg/L) for 72 h. Rapamycin (100 ng/L) was as a positive control. Cell cycle progress was determined by flow cytometry. Panel A shows representative histograms of flow cytometric analysis; Panel B is the summarized data of G₁ phase VSMCs after treatments. The percentage of G₁ phase cells in total events was calculated. Data are expressed as mean±SEM. n=5/group, One-way ANOVA. ^bP<0.05, ^cP<0.01 vs treatment control (0 mg/L matrine).