Figure 5.

Cytokine secretion of flagellated Salmonella SL5928(fliC/esat) through intranasal immunization. C57BL/6 mice were immunized intranasally with SL5928(fliC/esat), SL5928(fliC) or PBS. IFN-γ-producing and IL-4-producing cells in the lung, PPs, NALTs, spleen and MLNs were evaluated by ELISPOT assay with ESAT-6 peptide as the stimulus. Results were expressed as the mean number of spots per 10⁶ cells (six mice per group). **P<0.01 for the SL5928(fliC)-immunized and PBS-immunized mice using a Student's t-test. IFN, interferon; MLN, mesenteric lymph node; NALT, nasopharynx-associated lymph node; PBS, phosphate-buffered saline; PP, Peyer's patch.