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. 2014 May 7;9(5):e93364. doi: 10.1371/journal.pone.0093364

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© 2014 Liu et al

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Real-time PCR analysis of the expression of CAP, ALP, BSP and OPN in ADSCs grown in DFC-CM for 7 days. The expression levels were normalized to that of β-actin. The results represent mean values ( ± SD) from three independent experiments performed in triplicates. *P<0.05 vs. the control group. (B) CAP, BSP, and OPN were measured by Western blot in ADSCs grown in basal medium or in cells cultured in DFC-CM for 7 d. GAPDH was used as control for equal loading. (C) Quantitative analysis of the OPN, BSP, ALP and CAP. *P<0.05 vs. the control group.

Inline graphic — (A) Real-time PCR analysis of the expression of CAP, ALP, BSP and OPN in ADSCs grown in DFC-CM for 7 days. The expression levels were normalized to that of β-actin. The results represent mean values ( ± SD) from three independent experiments performed in triplicates. *P<0.05 vs. the control group. (B) CAP, BSP, and OPN were measured by Western blot in ADSCs grown in basal medium or in cells cultured in DFC-CM for 7 d. GAPDH was used as control for equal loading. (C) Quantitative analysis of the OPN, BSP, ALP and CAP. *P<0.05 vs. the control group.