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. 2014 May 7;9(5):e93364. doi: 10.1371/journal.pone.0093364

Figure 4. Wnt3a or DKK1 activate or inactivate the Wnt/β-catenin signaling pathway.

Figure 4

(A) The expression of β-catenin as well as GSK3β were measured by Western blot in ADSCs grown in DFC-CM or in basal medium (control) with or without the presence of Wnt3a (100 ng/mL)/DKK-1 (100 ng/mL) for 7 d. Beta-actin was used as control for equal loading. (B, C) Expression of LEF-1 (B) and TCF-4 (C) genes in ADSCs cultured in basal medium or in DFC-CM with Wnt3a (100 ng/mL)/DKK-1 (100 ng/mL) for 7 d. PBS was used as control condition. The expression levels were normalized to that of β-actin. The results represent mean values (SD) from three independent experiments performed in triplicates. *P<0.05 vs. the PBS group.