Table 2. Dynamic light scattering data.

Protein	Sample concentration (mg/ml)	R H (nm)	MW (kDa)	Pd (nm)	%Pd	Baseline	SOS Error (%)
WT-HiDapE	2	3.94	83.2	0.62	15.8	1.000	0.874
HiDapET	2	2.50	28.7	0.38	15.3	1.000	0.935
VcDapET	2	2.28	23.2	0.52	22.6	1.001	3.19

R _H – hydrodynamic radius; MW – molecular weight; Pd – the polydispersity, or width of the distribution, in nm, determined using a cumulants analysis (where the data are fit to an assumed distribution of particle sizes and the average radius and spread of radii is reported); %Pd – defined as Pd/R _H, the polydispersity divided by the estimated hydrodynamic radius from the cumulants fit of the autocorrelation function multiplied by 100; Baseline – The measured value of the normalized intensity autocorrelation curve. Values of 1.000 indicate that the measured correlation curve has returned to the baseline within the defined time. Deviations from the theoretical value of 1.000 typically indicate a noisy baseline; SOS error- the sum of squares difference between the measured correlation curve and the best fit curve calculated using the cumulants method of analysis, where a dust and noise free monomodal (single distribution) low polydispersity (narrow distribution) sample is assumed.