Figure 3. Genes and biological processes affected by Scap deletion in the developing lung.

A) Differentially expressed genes in Scap^Δ/Δ lungs were identified by mRNA microarray analysis. Heatmap analysis shows the two-dimensional clustering of 1079 mRNAs that were significantly altered in Scap^Δ/Δ mice compared with controls. The intensity in the red and green color ranges indicates increased versus decreased mRNAs, respectively. Each row represents a single mRNA, and each column represents an average fold change between knockout and control mice at the specified gestational age. B and C) Biological processes significantly influenced by epithelial Scap deletion were identified using ToppGene Suite (http://toppgene.cchmc.org). Enriched bioprocesses in genes induced (B) or suppressed (C) in Scap^Δ/Δ lungs are represented as red bars and green bars respectively. Statistical significance of each bioprocess was presented using negative log2 transformation of P value. D and E) qPCR analysis confirmed RNA microarray data demonstrating altered expression of genes involved in lipid biosynthesis/transport (D) and in lung development and maturation (E) in Scap^Δ/Δ mice (filled bars) compared with control (open bars), n = 3/group, *p<0.05.