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. Author manuscript; available in PMC: 2014 May 7.
Published in final edited form as: Matrix Biol. 2008 Jul 12;27(8):693–699. doi: 10.1016/j.matbio.2008.07.001

Fig. 1.

Fig. 1

(a) Diagram showing the position of the Cre recombinase in the BAC-Col10-Cre-frtNeofrt DNA. For the insertion of Cre, the lacZ cassette of the pLacH-Col10a1-LacZ-frtNeofrt vector (Gebhard et al., 2007) was replaced by Cre, and the resulting vector was recombined with BAC-Col10a1 (RP23.192A7) by homologous recombination in E. coli. Correct insertion into exon 2 of Col10a1 was controlled by PCR using primers P1 (Col10a1-Int1) and P5 (“cre reverse”). PCR using primers P1 and P4 served as wt control for endogenous Col10a1. (b) Pulse field gel electrophoresis of BAC Col10a1-LacZ-frtNeofrt DNA after linearization and molecular sieve chromatography on Sepharose 4B. 1) Pulse field markers 10–250 kb; 2)–6) fractions 3–7 of the Sepharose chromatography. Fractions 4 and 5 contain the majority of the BAC DNA which was used for microinjection. (c) Detection of BAC-Col10a1-Cre transgenic offspring by PCR using Primers P1 and P5; 1) markers 2) founder #1466 3) wt 4) Founder #1427; 5) BAC- Col10-Cre DNA.