Table 4. Residues from the active sites of β-glucosidases in direct contact with sector A positions.

Sector A position	Active site residues
	SfβGly	1UG6	1E4I	1E56	1V03	1E6S	2ZOX	3AHY
54	K201abr, M453abr	H178abr, A394abr	H179, A407	F205abr, F466abr	L203abr, S462abr	D201, N466	F179abr, N426	F179, A426
98	R97p	R75	R77	R96	R97	R95	R75	R73
143	H142bg, E187*, E190abr, K201abr	H119bg, E164*, C167, H178abr	H121bg, E166*, C168, H179	H142bg, D191*, T194, F205abr	H143bg, D189*, T192, L203abr	H141bg, Q187, S190abr, D201	H120bg, Q165*, F179abr	H119bg, E165*, C168, F179
188	E187*, E190abr	E164*, C167	E166*, C168	D191*, T194	D189*, T192	Q187, S190abr	Q165*, V168abr	E165*, C168
195	E194abr	C167, L171abr, H178	C168, L172, H179	T194, F198abr, F205abr	T192, V196abr, L203abr	S190abr, R194abr, D201	M172abr, F179abr	C168, P172, F179
196	E194abr	L171abr	L172	F198abr	V196abr	R194abr	M172abr	P172
203	E194abr, K201abr	H178abr	H179	F205abr	L203abr	D201	F179abr	F179
398	R97p, E399*	R75, E338*	R77, E352*	R96, E406*	R97, E404*	R95, E409*	E373*	R73, E367*
451	Q39bg, W444bg, M453abr	Q18bg, W385bg, A394abr	Q20bg, W398bg, A407	Q38bg, W457bg, F466abr	Q39bg, W453, S462abr	Q39bg, W457bg, N466	Q17, W417bg, N426	Q16bg, W417bg, N426
452	Q39bg, H142bg, K201abr, M453abr	Q18bg, H119bg, H178abr, A394abr	Q20bg, H121bg, H180, A407	Q38bg, H142bg, F205abr, F466abr	Q39bg, H143bg, L203abr, S462abr	Q39bg, H141bg, N466	Q17, H120bg, F179abr, N426	Q16bg, H119bg, F179, N426
460	Y331p, W444bg	Y284, W385bg	Y296, W398bg	Y333, W457bg	Y331, W453bg	W457bg	Y309, W417bg	Y298, W417bg

The numbering of the sector A positions was based on the Sfβgly sequence. * identifies catalytic glutamic acids; ^p – indicates residues involved in the modulation of the pK _a of the catalytic glutamic acids; ^bg – shows residues involved in the binding of the substrate glycone; ^abr – indicates residues that form the aglycone binding region. Data regarding the role of individual residues in substrate binding and catalysis were retrieved from the literature [5], [27], [28], [29], [9], [30]. β-Glucosidase from Spodoptera frugiperda Sfβgly; β-glucosidase from Thermus thermophilus (1UG6); β-glucosidase A from Paenibacillus polymyxa (1E4I); β-glucosidase Zmglu from Zea mays (1E56); β-glucosidase SbDhr from Sorghum bicolor (1V03); myrosinase from Sinapis alba (1E6S); Human cytosolic β-glucosidase (2ZOX);β-glucosidase from Trichoderma reesei (3AHY).