Figure 1. Experimental protocol.
Left anterior coronary artery occlusion (60 min) followed by reperfusion was produced in female mice; two days later, mice received 105 male murine CSCs (or PBS only) via intracoronary delivery. Heart, liver, lung, kidney, and spleen were collected at indicated time points. Each tissue was analyzed for the presence of donor CSCs by qPCR as described in Materials and Methods. In the 35-day group, hemodynamic measurements were performed prior to euthanasia to assess cardiac function.