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. 2014 May 7;9(5):e96904. doi: 10.1371/journal.pone.0096904

Figure 3. Sensitivity analysis of the allele-specific PCR assay.

Figure 3

(A) Sensitivity analysis using the bacterial genomic DNA. The detection limit was determined as 10 pg of bacterial DNA for E. coli serotypes O2 and O18 strains, and 500 pg of bacterial DNA for E. coli serotypes O1 and O78 strains, respectively. (B) Sensitivity analysis using the bacterial culture. The detection limit was determined as 10 CFUs of E. coli serotypes O2 and O18 strains, and 1,000 CFUs of E. coli serotypes O1 and O78 strains, respectively. Lane M: DL2000 Marker.