Table 1. Productivity levels and lipid and starch contents for axenic C. minutissima (top) and co-cultures of C. minutissima and E. coli (bottom).
| Culture conditions | Biomass productivity (mg/L/d) | Lipid productivity (mg/L/d) | Starch productivity (mg/L/d) | Final lipid content (%) | Final starch content (%) |
| Axenic C. minutissima | |||||
| Glucose | |||||
| 0 g/L | 8.4 (1.1) c | 2.0 (0.2) b | 0.09 (0.01) c | 23.5 (2.4) a | 1.04 (0.06) bc |
| 0.5 g/L | 38.0 (2.4) b | 5.1 (1.4) b | 0.30 (0.04) c | 13.4 (2.8) b | 0.78 (0.06) c |
| 2 g/L | 98.8 (4.9) a | 14.5 (1.3) a | 1.86 (0.68) b | 14.7 (0.6) b | 1.90 (0.68) b |
| 10 g/L | 98.7 (6.0) a | 13.5 (1.8) a | 3.16 (0.34) a | 13.7 (1.1) b | 3.19 (0.34) a |
| Glycerol | |||||
| 0 g/L | 13.5 (0.8) d | 2.8 (0.1) d | 0.27 (0.01) c | 20.8 (0.5) a | 1.98 (0.15) c |
| 0.5 g/L | 42.3 (1.5) c | 7.5 (0.4) c | 0.66 (0.04) c | 17.8 (1.5) a | 1.56 (0.05) c |
| 2 g/L | 111.0 (0.8) b | 19.6 (1.5) b | 4.04 (1.01) b | 17.7 (1.4) a | 3.65 (0.93) b |
| 10 g/L | 123.4 (0.9) a | 23.3 (1.8) a | 7.75 (0.78) a | 18.9 (1.6) a | 6.28 (0.67) a |
| Acetate | |||||
| 0 g/L | 19.9 (3.4) d | 3.3 (0.6) d | 0.20 (0.03) d | 16.6 (0.3) a | 1.03 (0.06) b |
| 0.5 g/L | 48.3 (2.3) c | 6.7 (0.6) c | 0.42 (0.02) c | 13.8 (0.6) b | 0.87 (0.02) c |
| 2 g/L | 125.8 (2.9) a | 18.5 (0.7) a | 0.84 (0.04) b | 14.7 (0.2) b | 0.67 (0.02) d |
| 10 g/L | 59.6 (3.9) b | 8.8 (0.8) b | 0.98 (0.03) a | 14.8 (1.3) b | 1.65 (0.06) a |
| C. minutissima, E. coli | |||||
| Glucose | |||||
| 0 g/L | 10.5 (1.8) d | 2.1 (0.3) d | 0.16 (0.02) d | 20.2 (0.4) b | 1.51 (0.04) bc |
| 0.5 g/L | 85.0 (3.5) c | 14.5 (1.1) c | 1.12 (0.05) c | 17.0 (0.9) c | 1.31 (0.02) c |
| 2 g/L | 190.8 (14.8) b | 25.9 (3.5) b | 3.48 (0.54) b | 13.6 (1.0) d | 1.67 (0.04) b |
| 10 g/L | 342.8 (65.6) a | 83.9 (15.8) a | 59.28 (9.70) a | 24.5 (1.4) a | 17.43 (2.11) a |
| Glycerol | |||||
| 0 g/L | 12.9 (4.9) d | 1.5 (0.5) c | 1.25 (0.50) c | 12.0 (1.0) b | 9.71 (0.58) b |
| 0.5 g/L | 74.7 (5.3) c | 9.5 (1.8) b | 3.79 (0.40) b | 12.6 (1.6) b | 5.07 (0.32) c |
| 2 g/L | 172.4 (15.5) b | 22.0 (2.5) b | 5.78 (2.50) b | 12.2 (0.5) b | 4.00 (0.11) c |
| 10 g/L | 419.8 (29.6) a | 72.2 (8.4) a | 76.97 (8.13) a | 17.2 (0.8) a | 18.31 (0.65) a |
| Acetate | |||||
| 0 g/L | 34.6 (2.5) d | 6.0 (0.1) d | 0.97 (0.06) b | 17.2 (0.8) a | 2.83 (0.36) b |
| 0.5 g/L | 87.5 (4.3) c | 13.4 (1.3) c | 1.06 (0.03) b | 15.3 (0.8) a | 1.22 (0.06) c |
| 2 g/L | 207.7 (1.4) b | 26.6 (1.1) b | 0.76 (0.11) c | 12.9 (0.5) b | 0.39 (0.05) d |
| 10 g/L | 428.9 (6.3) a | 72.5 (4.7) a | 26.52 (3.44) a | 16.9 (0.8) a | 6.18 (0.78) a |
Productivities are five-day averages and lipid and starch contents are a percent of dry weight. Three biological replicates were used in all cases except for co-cultures grown on 10 g/L glycerol where only two replicates were used. Mean value is followed by standard deviation in parenthesis. Within substrate batches (e.g. axenic C. minutissima w/glucose), values followed by the same letter are not significantly different at the 0.05 level.